Ultramicro determination of serum triglycerides by bioluminescent assay.

A simple, fully enzymic bioluminescent assay for triglycerides in serum.

In this fully enzymic bioluminescent assay, triglycerides are cleaved by lipase (EC 3.1.1.3) and carboxylesterase (EC 3.1.1.1), and the glycerol obtained is phosphorylated with ATP and glycerol kinase (EC 2.7.1.30). The ATP consumed in the latter reaction is determined by the firefly luciferin-luciferase reaction, and corresponds to the concentration of triglycerides. All the enzymic reactions ...

An ultramicro colorimetric method for determination of total and direct serum bilirubin.

A simple reproducible method for the ultramicro determination of total and direct serum bilirubin is presented. This method provides a greater sensitivity than that obtained with other colorimetric ultramicro procedures. Total bilirubin values agree with those obtained with the macro Malloy-Evelyn procedure, but direct-reading bilirubin values were significantly higher with the presented method.

Ultramicro Methods in Biochemistry. Vii. the Determination of Plasma or Serum Urea Nitrogen. 8. the Determination of Serum Uric Acid. Ix. the Determination of Serum Creatinine. Lf-22.

Ultramicro methods for the determination of serum urea nitrogen, uric acid, and creatinine have been evaluated and the results are re corded in reports VII, VIII, and IX, respectively. The method for the determination of urea nitrogen is an ultramicro adaptation of the reaction in which a blue color is developed when the ammonium ion reacts with phenol and hypochlorite. Ammonia is released from...

Adaptation of the Berthelot Color Reaction for the Determination of Urea Nitrogen in Serum and Urine to an Ultramicro System.

Optimized bioluminescence assay of creatine kinase and creatine kinase B-subunit activity.

Spectrophotometry of CK-B subunit activity based on immunoinhibition is rapid and convenient. However, the low sensitivity limits clinical applications to serum samples with CK-B activities considerably above normal. A sensitive, but not optimized, bioluminescent assay of CK-B, also based on immunoinhibition, has been described. We improved this method by the use of an ADP concentration saturat...